Table 1 Dissociation constants obtained for the different complexes. ^[a]SPR data were obtained as described in Fig. 3, with partner 2 (analyte) in solution and partner 1 (ligand) immobilized on the sensor chip surface. K_D values were determined using the Langmuir model. Errors on the K_D values were obtained according to: ΔK_D = K_D * (Δk_on/k_on + Δk_off/k_off). ^[b]ITC experiments were obtained as described in Fig. 2. Partner 2 injected from the syringe in the cell, containing partner 1. ITC data were treated using a model assuming equivalent and independent sites. ^[c]UCA experiments depicted in Figure S3. ^[d]FRET experiments depicted in Fig. 5. FRET intensities plotted as a function of total Ligand were fitted as described in Material and methods section. ^[e]Constants describing the intrinsic properties of the bidomains (K_{intra A3_A3} and K_{intra A3_bGFPD}) defined in Figure S5.

From: Ligand-induced conformational switch in an artificial bidomain protein scaffold