Figure 2
Addition of 2i broadly down-regulates UHRF1 and DNMT1 expression in various cancer cell lines. (a) Western blotting analysis showing addition of 2i down-regulated UHRF1 and DNMT1 proteins in A549, HeLa and KYSE70 cells. All the cells were cultured with or without addition of 2i for 2 days before harvested for western blotting analysis. (b) Western blotting analysis showing addition of 2i down-regulated UHRF1 and DNMT1 proteins in a panel of esophageal cells. (c,d) qRT-PCR analysis showing the effect of 2i on the levels of UHRF1 (c) or DNMT1 (d) mRNAs in various cancer cells. All the cells were cultured with or without addition of 2i for 2 days before harvested for qRT-PCR analysis. (e,f) Western blotting and qRT-PCR analysis showing the effect of 2i on the levels of UHRF1 and DNMT1 proteins (e) and mRNAs (f) in LNCaP, BEL7402, HepG2, C33A and SiHa cells. All the cells were cultured with or without addition of 2i for 2 days before harvested for western blotting and qRT-PCR analyses.
