Figure 5
Like PD0325901, the MEK/ERK inhibitor SCH772984 can also down-regulate the expression of UHRF1 and DNMT1 at the level of transcription. (a,b) HCT116 cells were treated with different concentrations of PD0325901 or SCH772984 before cells were harvested for western blotting analysis for ERK1/2 and phosphorylated ERK1/2 (a), 12 hours) and the levels of mRNAs for UHRF1, DNMT1 and DNMT3A (b), 2 days). (c) HCT116 cells were first treated with different concentrations of PD0325901 or SCH772984 for 36 h and were then treated without or with MG132 for 12 hours before harvested for analysis of the levels of proteins by western blotting using antibodies as indicated. (d,f) A549 (d) and KYSE70 (f) cells were first treated with different concentrations of PD0325901 or SCH772984 for 36 hours and were then treated without or with MG132 for 12 hours before cells were harvested for western blotting using antibodies as indicated. (e,g) A549 (e) and KYSE70 (g) cells were treated with different concentrations of PD0325901 or SCH772984 for two days before cells were harvested for qPCR analysis for the levels of mRNAs for UHRF1, DNMT1 and DNMT3A.
