Figure 5
From: Anoctamin-4 is a bona fide Ca2+-dependent non-selective cation channel
Endogenously expressed Ano4 is responsible for Ca2+-dependent cation conductance in RPE cells. (A) Confocal image of an ARPE-19 cell expressing Ano4. Staining with an antibody against Ano4 (green). Cell membrane was stained with ZO-1 (red). Scale bar represents 10 µm. (B) Raw currents through ARPE-19 cells before and after the application of ionomycin (1 µM). Application is indicated by the bar. (C) Current density-voltage plot before (filled circles) and after the application of ionomycin (open circles). (D) Comparison of the total current density in cells transfected with scRNA (black bar) and siRNA (grey bar). (E) Reversal potentials of the data shown in C before (black bars) and after (grey bars) the application of ionomycin. (F) Raw currents through ARPE-19 cells before and after the application of ATP (500 µM). (G) Current density-voltage plot before (filled circles) and after the application of ATP (open circles). (H) ATP-mediated increase in current density in cells transfected with scRNA (black bar) and siRNA (grey bar). The values are given as mean ± SEM. *p < 0.05; **p < 0.01.
