Figure 5
13 month old 3xTG mice have abnormal protein expression of phospho-tau (Ser202, Ser396 and total Tau5), PSD-95, NeuN, GFAP and ChAT in the cortex and/or HPC. (a) Representative western blots (male n = 4 per group, female n = 5 per group). Corresponding densitometric measurement in the (b) cortex (P-tau Ser202 genotype (F1,18 = 25.208, p < 0.0001); P-tau Ser396 genotype (F1,18 = 6.794, p = 0.021), sex (F1,18 = 5.855, p = 0.030), genotype x sex (F1,18 = 6.103, p = 0.027); PSD-95 sex (F1,18 = 5.876, p = 0.029), genotype x sex (F1,18 = 4.894, p = 0.044); NeuN genotype (F1,18 = 5.179, p = 0.039); GFAP sex (F1,18 = 10.885, p = 0.005), genotype x sex (F1,18 = 10.434, p = 0.006) and (c) HPC (P-tau Ser202 genotype (F1,18 = 77.628, p < 0.0001); tau5 genotype (F1,18 = 35.523, p < 0.0001); GFAP genotype (F1,18 = 23.200, p < 0.0001), sex (F1,18 = 7.261, p = 0.017), genotype × sex (F1,18 = 7.210, p = 0.018); ChAT sex (F1,18 = 6.468, p = 0.023), genotype × sex (F1,18 = 6.142, p = 0.027). All values for 3xTG mice are presented as a fold change in band intensity relative to Wt mice on the same blot; therefore, all Wt values are presented with a mean of 1. Data are mean expression ± SEM. ††p < 0.01 Indicates a significant difference between Wt and 3xTG (independent samples t-test, two-tailed, Bonferroni correction). ‡p < 0.05 Indicates a significant difference between males and females (independent samples t-test, two-tailed, Bonferroni correction). Blots have been cropped for conciseness; full length blots are presented in Supplementary Figures 5–8.
