Figure 10

The mineralized matrix of hDFSCs stained by alizarin red. Mineralized matrix formed by hDFSCs when incubated with various individual concentration (0, 10, 50 and 100 µg.mL⁻¹) of two nanosilicate (NS − F and NS + F) followed by culture in three different culture medium namely, NCM, OCM, and OIM for 14 d (a–u) and 21 d (A–U). Histograms (V–X) show the amount of alizarin red dye associated with mineralized matrix, formed by hDFSCs treated with nanosilicate particles (NS − F and NS + F) and cultured in three different medium NCM (V), OCM (W), and OIM (X). (one-way ANOVA followed by Tukey post-hoc *P < 0.05, **P < 0.01, ***P < 0.001).