Figure 4

Showing metabolic activity and morphology of hDFSCs after incubation with various concentrations of NS. The percent viability or metabolic activities of hDFSCs in the presence of different concentrations of NS cultured for 24 h (a), and 72 h (b). Changes in cellular morphology (actin filament arrangement) of hDFSCs when incubated for 48 h in presence of various concentration of NS namely, 0 µg.mL⁻¹ (c,g), 100 µg.mL⁻¹ (d,h), 1000 µg.mL⁻¹ (e,i) and 5000 µg.mL⁻¹ (f,j). Actin filament (red) stain by Alexa flour 546, and nuclei (blue) stained by DAPI. Scale bar is 100 µm.