Figure 4

Implementation of astigmatic 3D imaging in mmSTORM. The primary and secondary PSFs of Alexa Fluor dye molecules excited at 647 nm show circular and astigmatic shapes in a single image frame (a). The double disc structure of H-zone in sarcomeres has different visibility and length in the unfiltered (b) and in the STORM images reconstructed from two selected planes (c,d). The two optical sections (c,d) separated by 230 nm are marked in the schematic view of the tilted structure (e). Circles with a radius of 0.8 μm were fit to the cross-sections of zones 3 and 8 (f,g). The scale bar is 1 micron.