Figure 2

Resistance to C. higginsianum in BSR2-OX Arabidopsis lines. (a) Phenotypic responses to C. higginsianum inoculation. Three-week-old plants were inoculated with 105 conidia/mL of C. higginsianum. Unlike wild-type (WT; Col-0) plants, K02919 (a rice-FOX Arabidopsis T2 line; BSR2-OX) and Eil-0 (control ecotype resistant to C. higginsianum) plants were still green 13 d after inoculation. (b) Quantification of the relative fungal growth across plant lines. C. higginsianum Actin (ACT) DNA in the Arabidopsis plants were measured using quantitative real-time PCR 3 d after inoculation. The amounts of C. higginsianum genomic ACT DNA relative to Arabidopsis genomic ACT2 DNA in three independent BSR2-OX lines (K02919, RT:BSR2-OX#2 and RT:BSR2-OX#3) were significantly lower than those of WT plants (*P < 0.05 according to a t-test; n = 3–5; error bars represent the standard deviation).