Figure 3 | Scientific Reports

Figure 3

From: The rice CYP78A gene BSR2 confers resistance to Rhizoctonia solani and affects seed size and growth in Arabidopsis and rice

Figure 3

Screening rice-FOX Arabidopsis lines for resistance to R. solani. (a) Evaluation of R. solani resistance using the ratio of lesion length to leaf length. Six-week-old plants were used for drop inoculation with R. solani (MAFF243956; AG1-1A). Seven rice-FOX Arabidopsis lines showing resistance to both Pst DC3000 and C. higginsianum were used: RT:BSR2-OX#1 and #2, RT:AK101795-OX and RT:AK111775-OX are independent retransformed lines for AK072163, AK101795 and AK111775, respectively. K21617, K15424 and K04020 are independent transgenic rice lines with one rice cDNA insert for AK103699, BSR1 (AK070024) and AK066139, respectively. The lesion length and leaf length were measured 7 d after inoculation. Asterisks indicate that values are significantly different from the WT and vector control plants (***P < 0.001, according to a t-test; error bars represent the standard deviation; n = 3). (b) Representative Arabidopsis plants described in (a) immediately after inoculation and 7 d after inoculation. In comparison with the vector control and WT plants, the spread of disease symptoms stopped 7 d after inoculation in the BSR2-OX plants (RT:BSR2-OX#1 and #2). The arrows indicate the inoculation points. R, resistant; S, susceptible.

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