Figure 3

Tissue Clearing after Golgi-Cox and Original Golgi Staining. (a,b), 500 µm coronal brain section following Golgi-Cox impregnation (36 days) before clearing (a), and after clearing (b) overnight with modified CUBIC method. (c,d) Golgi-Cox-impregnated (29 days) whole hippocampus before (c) and after clearing (d) with modified CUBIC method overnight (d). (e,f) Cortical neurons after Golgi-Cox impregnation (43 days) and clearing for 24 h in CUBIC, imaged with a Nikon spinning-disk confocal microscope. (f) High magnification image of impregnated pyramidal neurons. (g,h) Original Golgi-impregnated 200 µm coronal brain section (g), and the same section after 24 h of CUBIC clearing (h). (I,j) Original Golgi impregnated whole hippocampus before CUBIC (i) and after 15 days of CUBIC protocol (j). (k,l), Original Golgi-stained, CUBIC-cleared hippocampus imaged with a Bioptonics OPT device, showing whole hippocampus tomography (k) and a high magnification of several granule cells (l). Scale bars: a–d, g–j = 1 mm; e, k = 200 µm, f = 40 µm, l = 100 µm.