Figure 4

Optimization of Golgi-Cox procedure in combination with CUBIC. (a,b) Golgi-Cox-impregnated 150 µm coronal brain section before (a) and after (b) CUBIC protocol without Kodak fixative. (c,d) Similar samples treated with Kodak fixative before CUBIC protocol, before (c) and after clearing (d). Note a substantially accelerated tissue clearing compared to (b). Scale bars: 100 = µm.