Figure 5
Golgi-Cox method and plaques staining in a mouse model of Alzheimer’s disease. (a) 200 µm coronal brain section of an APP knock-in mouse (APPNL-G-F) impregnated with the Golgi-Cox method and stained for amyloid-β plaques with thioflavin-S. Left, Golgi-Cox stain; middle, plaque stain; right, combined image, where plaques are shown in cyan. (b,c) High magnification of a z-projection image of an impregnated pyramidal cortical neuron and plaques (cyan). (c) Visualization of dendritic spines in the same impregnated cell as in (b), showing a plaque in very close proximity to a dendritic branch. Scale bars: a = 150 µm; b = 20 µm; c = 2 µm.
