Figure 7
Original Golgi for EM tracing. (a) A 100 µm brain section impregnated with the original Golgi method and followed by an extended gold toning for 60 min. The blue box indicates the ROI. (b) ROI excised from the specimen shown in (a), prior to imaging. (c) Block profile with the cropped section as in (a) after EM protocol, glued to a pin for serial BF-SEM. (d,e) The sample after 60 min of gold toning, imaged with LM (d), and serial BF-SEM (e). Blood vessels are visible as lighter areas indicated by black arrowheads. A distinctive region of a cell of interest is indicated by a white arrowhead. (f,g) High magnification TEM micrograph of an impregnated neuron. Note large sized, electron-dense deposits clearly masking some of the ultrastructure but providing much higher contrast as compared to Fig. 6f. (g) The size of gold particles allows to easily follow an impregnated neuron via a 3D volume and to fully reconstruct it, as shown in pink. Scale bars: a = 2 mm; b = 200 µm; d–e = 20 µm; f = 2 µm; g = 10 µm.
