Figure 3

Representative ITC results and fitting curves for (a) NADP⁺ binding to GoSLDH (100 μM); (b) NADPH binding to GoSLDH (100 μM) in the absence of NADP⁺; and (c) NADPH binding to GoSLDH (100 μM) in the presence of NADP⁺ (100 μM). ITC experiments were performed to characterize the binding thermodynamics of NADP⁺ to GoSLDH (100 μM) in 100 mM glycine-NaOH buffer (pH 10) at 25 °C.