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Figure 1

From: Imaging endogenous macrophage iron deposits reveals a metabolic biomarker of polarized tumor macrophage infiltration and response to CSF1R breast cancer immunotherapy

Figure 1The alternative text for this image may have been generated using AI.

Imaging and spatial profiling of tumor macrophage iron deposits ex vivo with iron MRI (FeMRI) and Prussian blue iron histology. (a) Representative Prussian blue iron histology of MMTV-PyMT TS1 mammary tumor cross-section. Scale bar 2.5 mm. Expansion shows iron+ macrophage deposits. Expansion scale bar 50 μm. (b) Representative ex vivo FeMRI of MMTV-PyMT TS1 mammary tumor cross-section. Scale bar 2.5 mm. Expansion shows high-iron pixel clusters. Expansion scale bar 50 μm. (c) Representative Prussian Blue iron histology of tumor associated macrophages (TAM), and (d) red blood cells (RBC). Note iron+ macrophages and iron− RBCs. (e) Iron deposit mask from Prussian blue histology. Scale bar 2.5 mm. (f) High-iron FeMRI cluster mask. Scale bar 2.5 mm. (g) Infiltration mapping using radial decile region rake sampling overlaid on high-iron MRI cluster mask. Scale bar 2.5 mm. 4x expansion shows high-iron FeMRI clusters and decile boundaries. (h) Infiltration profile showing number of histological iron deposits from Prussian blue (PB) and high-iron FeMRI pixel clusters (FeMRI) as a function of percent (%) infiltration into the MMTV-PyMT TS1 mammary tumors. (mean + s.e.m. n = 3 tissue cross-sections, n.s. p > 0.05 Kolmogorov-Smirnov test).

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