Figure 3 | Scientific Reports

Figure 3

From: Imaging endogenous macrophage iron deposits reveals a metabolic biomarker of polarized tumor macrophage infiltration and response to CSF1R breast cancer immunotherapy

Figure 3The alternative text for this image may have been generated using AI.

CD68 immunofluorescence and Prussian blue imaging of macrophage iron deposits in MMTV-PyMT murine breast cancer models of CSF1R immunotherapy. Representative CD68 macrophage immunofluoresent imaging in control and BLZ945 treated (a,b) TS1, and (c,d) 99LN tumors. Scale bar 250 µm (e) Absolute counts of CD68+ macrophages per mm2 MMTV-PyMT tumor cross-section in BLZ945 CSF1R inhibitor trials (mean + s.e.m. n = 4 mice/group, **p < 0.01, two-tailed unpaired students t-test). Representative iron staining using Prussian blue iron histochemistry in control and BLZ945 treated MMTV-PyMT (f,g) TS1, and (h,i) 99LN tumors. Scale bar 250 µm (j) Absolute counts of iron+ macrophages per mm2 tumor cross-section in the CSF1R inhibitor trials (mean + s.e.m. n = 4 mice/group, ***p < 0.001, two-tailed unpaired students t-test).

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