Figure 6 | Scientific Reports

Figure 6

From: Imaging endogenous macrophage iron deposits reveals a metabolic biomarker of polarized tumor macrophage infiltration and response to CSF1R breast cancer immunotherapy

Figure 6The alternative text for this image may have been generated using AI.

Spatial scores of murine tumor macrophage infiltration in CSF1R immune therapy trials from iron MRI and Prussian blue iron histology. (a) In vivo iron MRI (FeMRI) of MMTV-PyMT mammary tumor. Scale bar 1 mm. Expansion shows high-iron pixel clusters. Scale bar 100 µm. (b) Binary high-iron pixel cluster mask from FeMRI of MMTV-PyMT tumor cross-section. Scale bar 1 mm. Concentric rake region of interest grid overlay used to profile clusters is shown. Expansion shows detected clusters and concentric counting grid. Scale bar 200 µm. (c) Prussian blue iron stained cross-section of MMTV-PyMT tumor showing the rake grid overlay used to score the iron deposits. Scale bar 1 mm. (d) Expansion showing deposit, i.e. colony of iron+ macrophages. Scale bar 200 µm. Infiltration profiles of high-iron clusters from FeMRI in control and BLZ945-treated (e) TS1 and (f) 99LN MMTV-PyMT models (mean + s.e.m. n = 8 mice/group, *p < 0.05, ***p < 0.001, ****p < 0.0001, 2-way ANOVA with Sidak’s multiple comparison test). Iron+ macrophage deposit infiltration profiles from Prussian blue histology in control and BLZ945-treated (g) TS1 and (h) 99LN MMTV-PyMT models (mean + s.e.m. n = 4 mice/group, *p < 0.05, ***p < 0.001, ****p < 0.0001, 2-way ANOVA with Sidak’s multiple comparison test).

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