Figure 7
From: Novel carboxylate-based glycolipids: TLR4 antagonism, MD-2 binding and self-assembly properties
Dose-dependent inhibition of LPS-triggered TLR4 pathway activation in HEK293-Blue hTLR4 cells. Cells were pre-incubated with increasing concentrations (0.1 to 10 µM) of compounds FP13-17 in serum-free DMEM medium and stimulated with LPS (100 ng/mL) after 15 min. SEAP levels in cell culture media were quantified after 16 hours as indicator of TLR4 activation. Data were normalized to stimulation with LPS alone and fitted to a sigmoidal 4 parameter logistic equation to obtain dose-effect curves (left panel). IC50 values for FP13-17 are reported in the right panel. points represent the mean of percentage ± SEM of at least 3 independent experiments.
