Figure 9
From: Novel carboxylate-based glycolipids: TLR4 antagonism, MD-2 binding and self-assembly properties
Presence of serum or BSA neutralizes the antagonistic activity of monosaccharide FP13 on LPS-induced TLR4 signaling in RAW 264.7-Blue cells. A-C. Cells were pre-incubated with the indicated concentrations of monosaccharide FP13 in DMEM supplemented with 10% FBS (A), 4 mg/mL BSA (B) or 20 mg/ml BSA (C). 30 min later cells were stimulated with 1, 10 or 100 ng/mL E. coli O111:B4 LPS. SEAP reporter levels in the cell supernatant were determined by QuantiBlue Assay 24 h later. Error bars represent the standard deviation of the mean of biological triplicates. The graph is representative of at least three independent experiments.
