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Figure 1

From: Mitochondrial Dysfunction in Aged Macrophages and Lung during Primary Streptococcus pneumoniae Infection is Improved with Pirfenidone

Figure 1The alternative text for this image may have been generated using AI.

Mitochondrial Membrane Potential (ΔΨm) in Aged Lung Declines during S. pneumoniae Infection. Young (2 months) and aged (19 months) male and female BALB/c mice received 1 × 106 CFU of S. pneumoniae (ATCC 6303) or saline via intranasal instillation. Lung tissue from young and aged uninfected and S. pneumoniae infected mice was collected at select time points post infection. (A) ΔΨm was examined in lung cells by assessing changes in MitoCapture red and green fluorescence. Dot plots: young (red cells) and aged (blue cells). Histograms: young (black line) and aged (grey line). For histograms, N = 3 are shown for the 24–72 hour time points. (B) % of MitoCapture red positive cells and (C) mean fluorescence intensity (MFI) was assessed for each sample (t-test: *P < 0.05, **P < 0.01, and ****P < 0.0001). (D) DNA was isolated from uninfected and S. pneumoniae infected lung tissues and the ratio of mtDNA to nuclear DNA levels were assessed (t-test: ***P < 0.001 and ****P < 0.0001). (E) ATP concentration in young and aged lung was assessed at baseline and in response to infection (t-test: *P < 0.05). Similar results were obtained from at least two or more independent experiments with 10 mice per experiment. Data are expressed as the mean ± SD.

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