Figure 7
From: Crabp2 Promotes Metastasis of Lung Cancer Cells via HuR and Integrin β1/FAK/ERK Signaling
Crabp2 is inducible by tunicamycin and CRABP2 is correlated with CHOP in human lung tumors. (a) C10F4 cells were treated with dimethyl sulfoxide (DMSO, as control) or tunicamycin (3.5 or 5 μg/ml) for sixteen hours. Real-time RT-PCR was performed to analyze Crabp2 mRNA levels, and the statistical significance was determined using one-way ANOVA **p < 0.01, ***p < 0.001. (b) Western blot of Crabp2 and β-actin (ACTB, as the loading control) from C10F4 cells treated with DMSO (as control) or tunicamycin (3.5 or 5 μg/ml) for sixteen hours. (c,d) Immunohistochemical staining of CRABP2 as well as the cell stress marker CHOP in serial-sectioned lung tumor specimens (n = 59) (c). The results were assessed and scored by a pathologist, and the correlation between scores of CRABP2 and CHOP was analyzed by Spearman’s correlation test (Spearman r = 0.8299, p < 0.0001) (d). (e) Working hypothesis of Crabp2 regulates HuR and integrin β1/FAK/ERK signaling to promote metastatic behaviors.
