Table 1 Identification of enriched Fab sequences.

From: A rapid in vitro methodology for simultaneous target discovery and antibody generation against functional cell subpopulations

Antibody ID

Read count (+pool)

Enrichment score

AN01

579593

18.61

AN02

15347

13.37

AN03

12736

13.1

AN04

10027

12.75

AN05

5187

11.8

AN06

1579

10.09

AN07

1182

9.67

  1. Phage-Fab output pools from the positive enrichment and negative enrichment were collected after the fourth round of selection. Complementarity determining regions (CDRs) were PCR amplified and deep sequenced. Total number of reads in the positive (+) enrichment is shown in column 2, and column 3 shows the enrichment score calculated as: log2 positive read counts/log2 negative (−) enrichment read counts. See Table S1 for full list.
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