Figure 2
Difference in growth kinetics within the candidate cell lines. (a) Number of population doublings in 24 hours calculated in each consecutive passage indicates that two of the candidate cell lines demonstrated replicative senescence which is a classical hallmark of in vitro ageing (Cells were passaged to a density of 1:3 in each passage). (b) Quantification of average cell surface area in early and late passages of DPSC 44 and 45. Significance was determined by unpaired Student’s t-test; n = 70 cells per cell line were measured; ****p < 0.0001; Graph error bars are the means ± SEM (c) flow cytometric analysis of senescence-associated-β-gal activity in early and late passages of DPSC 44 and 45.
