Figure 2 | Scientific Reports

Figure 2

From: The ability to utilise ammonia as nitrogen source is cell type specific and intricately linked to GDH, AMPK and mTORC1

Figure 2

Glutamine synthetase and glutamate dehydrogenase protein abundance varies in different cell lines. Cells (HCT116, MCF10A, T47D, MCF7, HEK293 and Huh7) were plated in complete medium containing glutamine for 24 h. Cultures were lysed and collected for western blotting 24 h later. Levels of glutamine synthetase (GS) (A) and glutamate dehydrogenase (GDH) (B) relative to total protein (ponceau) were quantified using western blot. Note that MCF10A yielded very low amount of protein (total protein loaded ~4–16x more than other samples). (n = 3) One-way ANOVA p ≤ 0.001 demonstrates significantly different protein levels across samples, letters represent statistically distinct groups. Full-length blots are presented in Supplementary information Fig. 4

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