Figure 3
Proliferation in the presence or absence of ammonia in glutamine-depleted medium. HEK293 (A), Huh7 (B), T47D (C) and MCF7 (D) cells were plated and 24 h later they were washed with DPBS and cultured in fresh glutamine-depleted medium or glutamine-depleted medium with NH4Cl (n = 6). Cell proliferation was then measured based on live imaging using the Incucyte FLR assay over 7 days. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001.
