Figure 1

Expression of glycan antigens after DNA transfection of the 20 expression constructs into appropriate recipient cells analyzed by flow cytometry. After DNA transfection of cells with a variety of expression constructs and a vector expressing GFP or mRFP, cells were immunostained using anti-glycan antibodies, followed by flow cytometry. Average ( + SEM) values of the antigen-positive cell percentages (without adjustment to the original constructs) were calculated and are depicted as bar graphs. The construct names are shown at the bottom of the figure. Asterisks indicate the p values resulting from Student’s t-tests comparing each group with the empty vector control: ****P ≤ 0.0001, ***P ≤ 0.005, **P ≤ 0.01, and *P ≤ 0.05.