Figure 3 | Scientific Reports

Figure 3

From: Amino acid substitutions at sugar-recognizing codons confer ABO blood group system-related α1,3 Gal(NAc) transferases with differential enzymatic activity

Figure 3

FORS1 antigen expression was also observed of COS1(B3GALNT1) cells transfected with M_GBGT1(AlaGlyGly) or H_ABO-A(AlaGlyGly) using Lipofectamine 3000 and immunostained 2 days after DNA transfection. Whereas Fig. 2 shows the flow cytometry results of cells transfected with selected expression constructs using Lipofectamine 2000 and analyzed for FORS1 glycan expression 20 hours after DNA transfection, this figure shows those of cells transfected using Lipofectamine 3000 and analyzed 2 days after DNA transfection, employing the same immunostaining and flow cytometry protocols.

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