Figure 3

Secreted levels of C. difficile TcdA and TcdB are modulated by the spent media of defined microbial ecosystems. TcdA and TcdB levels of CD186 (a) and CD973 (b) were quantified after 24 and 48 h. Quantities shown are relative to the mean of matched vegetative cell counts for each sample. The mean of three replicate experiments are shown, with error bars showing the standard deviation observed. *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001; ****p ≤ 0.0001.