Figure 4
Targeted metabolic response of C. difficile CD186 (ribotype 027) after treatment with the spent media of defined microbial ecosystems. The net metabolite output of CD186 was determined by subtracting the mean metabolite concentration data of the microbial ecosystem spent medium from the metabolite data of CD186 treated with each defined microbial ecosystem spent medium after 24 h incubation. A biplot of the supervised clustering using PLS-DA (a) was used to visually determine the response of C. difficile to each ecosystem grouping. The biplot highlights the significant VIP scores displaying the metabolites that are important to the PLS-DA model (b). The net production of succinate (c) and isolecuine (d) by CD186 in response to defined microbial ecosystem spent media are shown. To determine statistical significance, a one-way ANOVA followed by Tukey’s HSD was used to correct for multiple comparisons when evaluating metabolite concentration data, and FDR adjusted p-values are reported. *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001; ****p ≤ 0.0001.
