Figure 5

PiT1 expression is induced by LPS in a dose-dependent manner. (A) RT-qPCR analysis of Pit1 mRNA expression in control BMDMs stimulated in vitro with 10 ng/ml LPS for the indicated times. Results were normalized to data from non-stimulated control cells. Data are means ± S.E.M. of three independent experiments. ANOVA test and Tukey’s multiple comparison test were performed. ^#Indicated comparison with untreated condition, ^###p < 0.001. (B) Representative western blot of PiT1 in BMDMs following treatment with 10 ng/ml LPS for the indicated times, with the corresponding quantification. (C) RT-qPCR analysis of Pit1 mRNA expression in control BMDMs stimulated in vitro with the indicated concentration of LPS for 16 h. Results were normalized to data from non-stimulated cells. Data are means ± S.E.M. of three independent experiments. ANOVA test and Tukey’s multiple comparison test were performed. ^#p < 0.05; ^##p < 0.01; ^###p < 0.001. (D) RT-qPCR analysis of Pit1 mRNA expression in WT MEFs following treatment with 100 ng/ml LPS treatment. Results were normalized to data from non-stimulated cells. Data are means ± S.E.M. of three independent experiments. ANOVA test and Tukey’s multiple comparison test were performed. ^#p < 0.05; ^##p < 0.01. (E) Representative western blot of PiT1 in MEFs following treatment with 100 ng/ml LPS for 4 h. Full length blots are presented in Supplementary Fig. 9.