Figure 7

PiT1-depletion is associated with impaired NF-κB activation. (A) Representative western blot of IκB degradation in control and PiT1-deficient BMDMs following LPS stimulation. BMDMs from Mx1-Cre; Pit1^lox/lox and control mice were stimulated in vitro with 10 ng/ml LPS for the indicated times (three independent experiments were performed). (B) Representative western blot of IκB degradation in WT and Pit1-KO MEFs following LPS stimulation (3 independent experiments were performed). MEFs were stimulated in vitro with LPS 100 ng/ml for the indicated times. (C) Representative western blot of p65 in nuclear (N) and cytoplasmic (C) extracts of WT and Pit1-KO MEFs following LPS stimulation. MEFs were stimulated in vitro with 100 ng/ml LPS for the indicated times. At least three independent experiments were performed. (D) Quantification of nuclear/cytoplasmic p65 ratio in WT and Pit1-KO MEFs. Mann-Whitney Rank Sum test was performed and significant differences between WT and Pit1-KO MEFs are indicated (*p < 0.05). Full-length blots are presented in Supplementary Fig. 9.