Figure 1 | Scientific Reports

Figure 1

From: Identification of Key Signaling Pathways Orchestrating Substrate Topography Directed Osteogenic Differentiation Through High-Throughput siRNA Screening

Figure 1

(A) Schematic workflow of the primary screen. (B) High-throughput siRNA library screen of 863 genes were distributed into designated wells in triplicates in 96 well plates. For each plate, SaOS2 cells grown in McKoy’s media (blue wells), SaOS2 cells grown in mineralization media (green wells), scrambled siRNA (orange wells), cell death siRNA (magenta wells), SaOS2 cells with only secondary Ab (yellow well) and only secondary Ab (red well) wells were used as internal controls. Three 96 well plate replicate was used. (C) Representative ICW images for Cell death, Scrambled, Mineralization Media Control, A test gene that upregulated osteogenesis, A test gene that downregulated osteogenesis, A test gene that has no effect osteogenesis compared to control. White circles represent the region of interest for quantification of RUNX2 ICW signal.

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