Figure 4
From: An ADAMTS Sol narae is required for cell survival in Drosophila
Negative correlation of sona and diap1 patterns is accentuated by irradiation. (a,b) Patterns of Diap1 and LacZ in control (a) and sona > lacZ wing discs irradiated at 4,500 rad (b). Arrowheads mark the region with negative correlation, a high level of Diap1 and a low level of LacZ (a’,a”). (c,d) Patterns of Diap1-lacZ and GFP in control (c c’) and irradiated sona > GFP; Diap1-lacZ wing discs at 4,500 rad (d,d’). (e,f) The charts display the expression level between sona (LacZ) and Diap1 in control (e) and irradiated sona > lacZ wing discs at 4,500 rad (f). (g,h) The 2D histograms show correlation between sona (LacZ) and Diap1 values in the discs before (g) and after irradiation (h). (i,j) Basal plane of the irradiated sona > lacZ disc. The white box in i is magnified in j. Arrow mark CC3 signals in the cell with no Diap1, and arrowheads mark the cell with sona but no CC3 signals. (k–m) The pattern and level of rpr-LacZ and sona (GFP) in control (k) and in irradiated (l) in rpr-lacZ, sona > GFP discs. The region marked with a white box in l is magnified in m. Cells with no GFP, but with a high level of LacZ are marked with yellow arrows, and cells with a high level of GFP, but with no LacZ are marked with white arrows. Scale bars: (a–d,i) 60 μm; (j) 15.4 μm; (k,l) 40 μm; (m) 5.7 μm.
