Figure 4

Simultaneous immunogold labelling of (a) wild type and (b) Tmc1−/− OHC bundles from apical cochlear regions, and (c) gold particle counts (mean ± SEM, n = 3) from both at P10. Virtually no labelling was detected on Tmc1−/− bundles whereas labelling on wild type was present as before at shorter stereocilia tips (arrow) and on the apical membrane (arrowheads). The mean gold particles per bundle was significantly different between the two [Wilcoxon (Mann-Whitney) test, p < 0.05].