Figure 3 | Scientific Reports

Figure 3

From: Correlative cryo super-resolution light and electron microscopy on mammalian cells using fluorescent proteins

Figure 3

Deactivation of fluorescent proteins at 294 K and 77 K. The first deactivation curve of traces shown in Fig. 2 were fitted with a (bi-)exponential function, of which the fast rate is plotted in (a) and (b) for 294 K (aqueous) and 77 K (vitreous) respectively. (c) and (d) Deactivation prior to vitrification of FPs leads to retention of the deactivated state for rsFastLime and rsEGFP2, respectively. Following vitrification, fluorescent intensity was measured after 0 and 7 days, and the mean intensity before (dark grey) and after (light grey) a photoactivation pulse are shown. Deactivation for 20 min after vitrification of the FPs is shown, as well as photoactivation without any deactivation.

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