Figure 4
CryoCLEM using rsEGFP2-labelled lipid nanotubes. (a) Diffraction-limited cryoFLM image of a single lipid nanotube labelled with rsEGFP2. (b) SR image of the same region. The colourmap represents the number of localised single-molecules per SR pixel. (c) CryoEM image of the single tube overlaying a hole in the carbon support film. (d) Intensity profiles along the tube for each modality shown in (a–c). Multiple profiles were averaged perpendicular to the tube, indicated by the yellow box and lines in (c). (e) and (i) Diffraction-limited cryoFLM image of several bundled nanotubes labelled with rsEGFP2. (f) and (j), SR image of the same regions shown in e and i, respectively. (g) and (k), CryoEM image of the same regions SR image of the same regions shown in e and i, respectively. (h) and (l), Intensity profiles across the yellow line shown in (e–g) and (i–k). Profiles are drawn such that the yellow sphere corresponds to the left hand side in the profile plot. Scalebars: 1 µm.
