Figure 5
CryoCLEM on U2OS cells, transfected with rsEGFP2-MAP2. (a) Diffraction-limited cryoFLM image, (b) SR image and (c) CryoEM image of the same region, showing two positively transfected cells. (d–f) Higher magnification images of the region indicated with the white square in a-c, diffraction-limited cryoFLM, SR image and cryoEM image respectively. (g) Overlay of the SR image shown in e over an 18.6 nm thick slice through a tomographic volume of the sample in the same region. (h) An 18.6 nm thick slice through the tomographic volume of the sample in the same region as shown in (f), where the microtubule bundles can be clearly distinguished. Scalebars: 10 µm, in (a–c); 1 µm in (d–h).
