Figure 2
Introduction of smvA into E. coli leads to increased resistance to chlorhexidine. MIC values to cationic biocides (A) and growth profiles (B) for E. coli expressing plasmids containing smvA or smvAR from S. enterica or K. pneumoniae. For E. coli clones KP indicates the fragment was cloned from K. pneumoniae and ST from S. enterica serovar Typhimurium. smvAR indicates that the fragment contains full length smvA and smvR including their promoters and smvA indicates that the fragment contains a full length smvA including its promoter. C2 indicates clone number 2 etc. Coding mutations found in various fragments are indicated. Control indicates plasmid only. Growth curves are the means of three independent repeats and statistical significance between clones and the control is shown where appropriate. All MIC values are given as mg/L except CET (%). For abbreviations see Table 1.
