Table 3 Variants in SmvAR and their effect on biocide tolerance.

From: SmvA is an important efflux pump for cationic biocides in Klebsiella pneumoniae and other Enterobacteriaceae

Strain

Biocide MIC (mg/L)

SmvAR status

CHD

CET*

OCT

CPC

CTAB

HDPCM

SmvA

SmvR

Enterobacter

BS35

16

0.0039

4

32

32

16

WT

C-terminal Extension

BS92

8

0.0009

2

8

16

16

WT

WT

CFI_002_CRCN

2

≤0.0005

2

8

8

8

Absent

Absent

CFI_004_KPC

1

≤0.0005

1

4

4

2

Disrupted

WT

Salmonella

FWE 1401

8

0.0039

2

16

32

16

WT

WT

FWE 1401 CHD

512

0.07

2

32

64

16

WT

Insertion

Citrobacter

CK 51

2

0.0009

1

1

8

4

Disrupted

WT

CK 54

2

0.0009

1

1

8

4

Disrupted

WT

NCTC 9750

8

0.0009

2

2

8

8

WT

WT

NCTC 9750 CHD

32

0.0019

2

2

16

8

WT

Mutation

8

32

0.0039

2

2

32

16

WT

WT

8 CHD

256

0.015

4

4

64

32

WT

Transposon insertion

K. pneumoniae

BS26

8–16

≤0.0005

4

4

8

4

Disrupted

WT

M403

≤0.5

≤0.0005

2

4

8

1

Absent

Absent

M635

≤0.5

≤0.0005

2

4

8

1

Absent

Absent

M665

≤0.5

≤0.0005

2

2

4

2

Absent

Absent

KP 115

1

0.0019

2

4

16

4

Absent

Absent

6

32

0.0009

4

4

16

4

WT

WT

6 CHD

128

0.007

4

64

128

64

WT

Deletion

19

32

0.0018

4–8

16

32

16

WT

WT

19 CHD

64

0.0018

8

16

32

16

WT

Mutation

20

32

0.0009

8

4

8

4

WT

WT

20 CHD

64

0.003

4–8

8

16

32

Mut in prom

Mut in prom

K. oxytoca

0552

4

0.007

4

8

32

16

Absent

Absent

7227

16

0.015

4

8

128

16

WT

WT

7227 CHD

64

0.015

8

32

128

64

Mut in prom

Mut in prom

  1. WT indicates that the sequence is full length. For list of abbreviations see Table 1. CHD indicates strains which have been adapted to chlorhexidine. *Value is given as %.
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