Figure 5
Dilution Series of a Single Target. Panel (a) shows the PCR curves from a dilution series of Parainfluenza Virus 3 from 105 down to 50 copies/sample, with 6 replicates at each concentration. The tight grouping among replicate curves indicates that our assay should produce consistent CT values from run to run. Panel (b) confirms this by showing the average CTs of the replicates at each concentration relative to a standard at 104 copies, along with 1-σ error bars. Not only do the small error bars indicate tight grouping, but the points are well matched to a linear fit of CT vs. log(Concentration). This indicates that quantitation could be potentially viable when at most one target amplifies per color channel.
