Figure 6
Illustration of Amplitude Modulation Coding. (a) Traditional PCR amplification. The low ratio of probe to primer concentration causes all probe to be digested over the course of amplification, resulting in an fluorescent signal proportional to the initial probe concentration. (b) The intensity of the output signal from PCR can be modulated by altering the initial concentration of probe present in the reaction. (c) By adding probes for multiple different targets to the reaction at increasing concentrations, we can make each combination of targets correspond to a unique total amount of probe. This causes each combination of targets to produce a unique total fluorescent signal at the end of amplification.
