Figure 5

Selection of iPSCs with high pluripotency based upon the PD index. (a) Scheme of isolation and data collection of iPSC clones. MEFs harboring the GFP reporter gene on an inactive X chromosome were reprogrammed by the SeVdp(KOSM) vector, and colonies were cloned randomly at day 21. (b) PD indices of isolated iPSC clones. Data represent means ± SEM of the PD indices obtained from at least five images. (c) GFP fluorescence in isolated iPSC clones described in (b). GFP fluorescence of each iPSC clone was observed by phase contrast fluorescence microscopy. Scale bars, 100 µm. (d) Relationship between the GFP fluorescence and PD index. The intensity of GFP fluorescence in each iPSC clone was quantified using AxioVision software.