Table 1 Identified up-regulated and differentially regulated proteins (pH 3–10) in highly aggressive Tilletia indica isolate TiK grown in the presence of host extract by MALDI-TOF/TOF.
Spot | Protein | Accession | Organism | Function | Exper. pI/Mr | Theo. pI/Mr | MASCOT Score | No. of Peptides |
|---|---|---|---|---|---|---|---|---|
1 | 1,4-benzoquinone reductase | OAJ02041.1 | Tilletia caries | Involved in lignin degradation. | 7.25/22.35 | 6.70/22.00 | 141 | 4 |
2 | Hypothetical protein | EYE93962.1 | Aspergillus ruber | — | 7.14/28.24 | 7.11/27.14 | 84 | 5 |
3 | Salicylate hydroxylase | OJJ80335.1 | Aspergillus glaucus | Degrade plant produced Salicylic acid and suppress plant defense response. | 6.57/45.34 | 5.90/46.51 | 82 | 5 |
4 | Hypothetical protein | EQK97475.1 | Ophiocordyceps sinensis | — | 6.75/45.33 | 6.60/45.32 | 82 | 10 |
5 | S-adenosylmethionine synthetase | OAJ18635.1 | Tilletia walker | Involved in development, stress response. | 6.84/45.34 | 5.95/42.49 | 122 | 6 |
6 | Isocitrate lyase | GAT26921.1 | Aspergillus luchuensis | Involved in cuticle penetration and appressorium formation. | 6.80/40.54 | 6.30/59.70 | 82 | 7 |
7 | Isochorismatase hydrolase | KRH93850.1 | Verticillium dahlia | Degrade Isochorismate, precursor of salicylic acid biosynthesis. | 6.52/40.52 | 6.45/84.11 | 92 | 14 |
8 | Isocitrate lyase | GAT26921.1 | Aspergillus luchuensis | Involved in cuticle penetration and appressorium formation. | 5.50/29.54 | 6.30/59.70 | 85 | 8 |
9 | Isochorismatase hydrolase | KRH93850.1 | Verticillium dahlia | Degrade Isochorismate, precursor of salicylic acid biosynthesis. | 5.00/29.54 | 6.45/84.11 | 82 | 11 |
10 | Hypothetical protein | OAL35951.1 | Fonsecaea nubica | — | 4.50/29.00 | 4.38/29.43 | 115 | 2 |
11 | Isochorismatase hydrolase | KRH93850.1 | Verticillium dahlia | Degrade Isochorismate, precursor of salicylic acid biosynthesis. | 5.83/53.45 | 6.45/84.11 | 90 | 11 |
12 | Hypothetical protein | XP_008716200.1 | Cyphellophora europaea | — | 7.25/36.00 | 5.13/344.20 | 84 | 15 |
13 | Hypothetical protein | ORX65063.1 | Linderina pennispora | — | 6.15/45.40 | 10.18/393.28 | 100 | 25 |
14 | Cys4P | AJR00164.1 | Saccharomyces cerevisiae | Involved in Cystathionine biosynthesis. | 8.13/36.15 | 6.25/56.08 | 82 | 15 |
15 | Hypothetical protein | ODQ74899.1 | Lipomyces starkeyi | — | 8.25/54.50 | 8.20/136.32 | 81 | 13 |
