Table 2 Identified up-regulated and differentially regulated secretory proteins (pH 3–10) in highly aggressive Tilletia indica isolate TiK grown in the presence of host extract by MALDI-TOF/TOF.
Spot | Protein | Accession | Organism | Function | Exper. pI/Mr | Theo. pI/Mr | MASCOT Score | No. of Peptides |
|---|---|---|---|---|---|---|---|---|
1 | Glycosyl hydrolase 30 | OAJ15739.1 | Tilletia walkeri | Possess endo-β-1,4-xylanase activity. | 6.85/64.43 | 8.38/51.67 | 264 | 11 |
2 | Hypothetical protein | XP_007345262.1 | Auricularia subglabra | — | 5.14/24.00 | 5.16/44.05 | 71 | 12 |
3 | Peroxiredoxin | ORY18094.1 | Neocallimastrix californiae | Reduces H2O2 and protect pathogen against host generated reactive oxygen species. | 5.25/19.85 | 7.60/21.12 | 89 | 13 |
4 | Aspartyl protease | OAJ21989.1 | Tilletia walkeri | Degradation of host cell wall proteins and antifungal proteins. | 4.25/29.08 | 5.82/41.92 | 171 | 10 |
5 | Related to stress response protein rds1p | OAJ21332.1 | Neurospora crassa | — | 4.62/36.00 | 4.93/31.31 | 117 | 5 |
6 | Hypothetical protein | XP_002487729.1 | Talaromyces stipitatus | — | 5.24/36.17 | 8.99/77.28 | 84 | 15 |
7 | Hypothetical protein | KJK82765.1 | Metarhizium anisopliae | — | 5.56/48.34 | 7.22/440.83 | 124 | 38 |
8 | Hypothetical protein | OAJ20939.1 | Tilletia walkeri | — | 5.06/81.72 | 4.75/94.02 | 111 | 16 |
9 | Hypothetical protein | KFY26206.1 | Pseudogymnoascus spp. | — | 5.12/75.64 | 5.25/30.93 | 121 | 24 |
10 | Dual specificity phosphatase | OQD63390.1 | Penicillium polonicum | Phosphatase activity | 5.54/54.50 | 6.01/38.22 | 86 | 9 |
11 | Dual specificity phosphatase | OQD63390.1 | Penicillium polonicum | Phosphatase activity | 5.54/54.48 | 6.01/38.22 | 86 | 9 |
12 | Hypothetical protein | KIK53675.1 | Gymnopus luxurians | — | 5.00/44.56 | 6.02/104.535 | 136 | 24 |
13 | Hypothetical protein | OBT79903.1 | Pseudogymnoascus spp. | — | 5.12/40.00 | 7.24/71.06 | 89 | 16 |
14 | Serine/threonine protein kinase | ENH85273.1 | Colletotricum orbiculare | Kinase activity | 5.20/48.50 | 5.92/70.80 | 105 | 11 |
15 | Uncharacterized protein | KLO88873.1 | Fusarium fujikuroi | — | 5.20/48.43 | 10.71/15.57 | 90 | 9 |
16 | Fungal specific transcription factor | OAQ74759.1 | Purpureocillium lilacinum | Transcription factor - | 5.50/43.03 | 5.92/58.63 | 83 | 10 |
17 | Fungal specific transcription factor | OAQ74759.1 | Purpureocillium lilacinum | Transcription factor | 5.50/42.78 | 5.92/58.63 | 83 | 10 |
18 | Hypothetical protein | KHE85708.1 | Neurospora crassa | — | 6.20/28.60 | 6.30/22.71 | 95 | 15 |
19 | Tagatose bisphosphate aldolase | KIR55487.1 | Cryptococcus gattii | Convert Tagatose-1,6- bisphosphate to glycerone phosphate | 7.10/2412 | 5.14/31.72 | 71 | 6 |
