Figure 3 | Scientific Reports

Figure 3

From: Interplay between lipid lateral diffusion, dye concentration and membrane permeability unveiled by a combined spectroscopic and computational study of a model lipid bilayer

Figure 3

(a) Mean square displacement (MSD) evaluated as a function of time from MD simulations of DOPC (black, DOPC lipids), RHB-DOPC (red, RHB lipids) and RHBAgg-DOPC (blue, RHBAgg lipids) lipid bilayers. Error bars correspond to standard errors. Differences in error bars reflects mainly the different number of systems (i.e., 400 DOPC lipids, 8 free RHB lipids, 1 RHB aggregate). (b) Mean square displacement (MSD) evaluated as a function of time from MD simulations of DOPC (black, DOPC lipids), RHB-DOPC (red, DOPC lipids) and RHBAgg-DOPC (blue, DOPC lipids) lipid bilayers. Error bars correspond to standard errors. (c) Schematic representation of a FCS measurement. (d) Confocal microscopy image of a GUV DOPC − 0.1% RHB, scale bar 1 micron. (e) Effect of fluorophore concentration on the diffusion of RHB lipids in GUVs. (f) Emission spectra of Rhodamine B at different concentrations (0.1% and 10%) in GUVs, the aggregation of Rhodamine B induces an emission spectra red shift of 8 nm, from 583 to 591 nm.

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