Table 3 Results of PCR amplification for the ompA, ompB, htrA, sca4 and 16S rRNA genes.

From: Diversity of spotted fever group rickettsiae and their association with host ticks in Japan

gltA genotype

Tick species

No. tested

Mean rickettsial burden (copies/µl)*

PCR amplification

ompA

ompB

htrA

sac4

16S rRNA

G1

H. megaspinosa

2

7.9E + 3

−

 + 

 + 

 + 

 + 

G2

H. hystricis

2

1.1E + 4

−

 + 

 + 

 + 

 + 

G3

I. persulcatus

4

8.7E + 3

 + 

−

 + 

−

 + 

G4

I. persulcatus

3

8.4E + 3

−

 + 

 + 

−

 + 

G5

I. persulcatus

6

1.3E + 3

−

−

+

−

−

G5

I. monospinosus

6

2.3E + 2

−

+

+

+

+

G6

H. longicornis

2

2.4E + 3

+

+

+

+

+

G7

H. formosensis

1

1.0E + 4

+

+

+

+

+

G8

A. testudinarium

3

2.1E + 4

+

+

+

−

+

G9

H. kitaokai

2

1.6E + 4

−

+

+

−

+

G10

I. nipponensis

2

3.0E + 3

+

+

+

−

+

G11

H. japonica

3

2.6E + 3

−

+

+

+

+

G11

H. flava

7

1.8E + 3

−

−

+

−

−

G12

H. flava

3

1.2E + 3

−

−

+

−

−

G13

I. ovatus

5

2.5E + 3

−

+

+

−

+

G14

H. formosensis

3

3.6E + 3

−

−

+

−

−

G15

H. formosensis

3

1.3E + 3

−

−

+

−

−

  1. +, Amplified; −, Not amplified. *The mean copy number of rickettsial gltA gene in the template DNA was calculated by gltA real-time PCR.