Table 4 Primers uesd in the present study.
From: Diversity of spotted fever group rickettsiae and their association with host ticks in Japan
Primer | Sequence (5′-3′) | Target gene | Annealing temperature (°C) | Amplicon size (bp) | Reference |
|---|---|---|---|---|---|
CS-F | TCGCAAATGTTCACGGTACTTT | citrate synthase gene (gltA) | 60 | 74 | Steno et al.43 |
CS-R | TCGTGCATTTCTTTCCATTGTG | Â | Â | Â | Â |
CS-P | TGCAATAGCAAGAACCGTAGGCTGGATG | Â | Â | Â | Â |
gltA_Fc | CGAACTTACCGCTATTAGAATG | citrate synthase gene (gltA) | 55 | 580 | Gaowa et al.16 |
gltA_Rc | CTTTAAGAGCGATAGCTTCAAG | Â | Â | Â | Â |
Rr.190.70p | ATGGCGAATATTTCTCCAAAA | outer membrane A gene (ompA) | 48 | 542 | Regnery et al.44 |
Rr.190.602n | AGTGCAGCATTCGCTCCCCCT | Â | Â | Â | Â |
120_2788 | AAACAATAATCAAGGTACTGT | outer membrane B gene (ompB) | 48 | 816 | Roux and Raoult45 |
120_3599 | TACTTCCGGTTACAGCAAAGT | Â | Â | Â | Â |
17k_5 | GCTTTACAAAATTCTAAAAACCATATA | 17-kDa common antigen gene (htrA) | 52 | 550 | Labruna et al.47 |
17k_3 | TGTCTATCAATTCACAACTTGCC | Â | Â | Â | Â |
Rick_16S_F3 | ATCAGTACGGAATAACTTTTA | 16S ribosomal RNA gene (16S rRNA) | 52 | 1328 | Anstead et al.48 |
Rick_16S_F4 | TGCCTCTTGCGTTAGCTCAC | Â | Â | Â | Â |
rrs2_seq_1 | AGGCCTTCATCACTCACTCG* | Â | Â | Â | This study |
rrs2_seq_2 | CTACACGCGTGCTACAATGG* | Â | Â | Â | Â |
D1f | ATGAGTAAAGACGGTAACCT | surface cell antigen-4 (sca4) | 50 | 928 | Sekeyova et al.46 |
D928r | AAGCTATTGCGTCATCTCCG | Â | Â | Â | Â |
sca4_seq_1 | GCCGGCTATTTCTATTGATTC* | Â | Â | Â | This study |
sca4_seq_2 | TGCAAGCGATCTTAGAGCAA* | Â | Â | Â | This study |
