Figure 1

Behavior analyses of zCry1a KO, zPer2 KO, and DKO zebrafish. (A) Schematic representation of the genomic structures of the zCry1a and zPer2 genes. Exon regions are shown as solid boxes. Arrows indicate the exons where nonsense mutations were introduced. (B) Total locomotor activities of WT, zCry1a KO, zPer2 KO, and DKO zebrafish were calculated from day 6 to day 8. Zebrafish were raised in constant dark (DD) conditions for 5 days after fertilization, and then a 3 h light pulse was administered at the beginning of 6 day after fertilization. Their moving distance was then analyzed in DD condition for 4 days. P < 0.05 (Student’s t-test). [WT (n = 11), zCry1a KO (n = 11), zPer2 KO (n = 10), DKO (n = 11)]. (C) Total locomotor activities of WT, zCry1a KO, zPer2 KO, and DKO zebrafish were calculated from day 7 to day 9 after fertilization. Zebrafish larvae were raised in DD conditions for 5 days after fertilization, and then a 12 h light pulse was administered at the beginning of 6 dpf. Their moving distance was then analyzed in DD condition from 7 to 10 days after fertilization. P < 0.05 (Student’s t-test). [WT (n = 12), zCry1a KO (n = 11), zPer2 KO (n = 11), DKO (n = 11)] (D) Representative double-plotted activity records (actograms) of WT, zCry1a KO, zPer2 KO, and DKO zebrafish analyzed in B. Note that the range of Y axis of the actogram for DKO animal is distinct from those for WT, zCry1a KO, and zPer2 KO zebrafish. (E) Representative double-plotted activity records (actograms) of WT, zCry1a KO, zPer2 KO, and DKO zebrafish analyzed in C. Note that the range of Y axis of the actogram for DKO animal is distinct from those for WT, zCry1a KO, and zPer2 KO zebrafish.