Figure 5 | Scientific Reports

Figure 5

From: The clock components Period2, Cryptochrome1a, and Cryptochrome2a function in establishing light-dependent behavioral rhythms and/or total activity levels in zebrafish

Figure 5

Characterization of cellular clocks in WT and TKO cells at the single cell level. (A) WT, DKO, and TKO cultured cells were transfected with the Per1::Luc construct and exposed to light for 3-h or 12-h. Per1 reporter bioluminescence in each cultured cell was monitored over the indicated time course. The bioluminescence values were detrended according to the instrument protocol and the detrended values were normalized by averaging intensity over time. Detrended data representative of three independent experiments are shown. (B) Left panels: Luminescent traces from individual WT and TKO cultured cells maintained in DD conditions (Dark) or exposed to light for 12-h (Light) were monitored by real-time bioluminescence imaging. [WT dark (n = 24), WT light (n = 24), TKO dark (n = 23) TKO light (n = 25)] Right panels: Circular histograms of phase distribution from WT or TKO cultured cells kept in DD conditions (Dark) or exposed to 12 h light (Light). The times having peak signal levels were determined using the “Cosinor” and “Acro” software.

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